ISSN: 2456–5474 RNI No.  UPBIL/2016/68367 VOL.- VIII , ISSUE- VII August  - 2023
Innovation The Research Concept

Anti-fungal and Bacterial analysis of compounds 4-(2-chloro-6-substitutedquinolin-3-y1)-3-chloro-1-{443-(4-substituted phenyl)- acrylolyll-phenyl}-azetidine-2-ones
Paper Id :  18015   Submission Date :  22/08/2023   Acceptance Date :  22/08/2023   Publication Date :  25/08/2023
This is an open-access research paper/article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
For verification of this paper, please visit on http://www.socialresearchfoundation.com/innovation.php#8
Kunwarvir Singh
Assistant Professor
Chemistry
CMIT College
Bulandshahar,Uttar Pradesh, India
Mohit Kumar
Research Scholar
Chemistry
GLA University
Mathura, Uttar Pradesh, India
Abstract The newly synthesized final compounds were analysed for their work in vitro antibacterial and antifungal activities against many strains Fungas and Bacteria. Preliminary results was Found and indicated that most of the compounds performed/demonstrated very good antimicrobial activity. The synthesis derivatives(4-(2-chloro-6-substituted¬quinolin-3-y1)-3-chloro-1-{443-(4-substituted phenyl)- acrylolyll-phenyl}-azetidine-2-ones) were screened for antibacterial, antifungal, and antiacetylcholinesterase activities in vitro
Keywords Anti-fungal, Bacterial Analysis.
Introduction

Thus a large variety of organic compounds like quinones, Schiff basesi°, phenols organometallic comp. organophosphorus11-13 group of compound exhibit diverse biological activity like fungicides, insecticides14 pesticidesI5-16 plant growth regulatois17 antibiotic's, enzymatic aldolizationl° anti-cancer agents2° and oxygen carriers in biological systems. Keeping the above facts in mind. It has been decided to study the anti-bacterial activity of synthesized new chalcones. Common types of bacteria are given non-scientific names according to their shapes, cell groupings, ability to form spores and reaction to some special stains. The most important of these special method of staining is the gram stain. It is a differential stain, since it colors some bacteria the original purple color and others the color of the counter stain, usually pink. Those that keep the purple stain are called gram positive and those take the counter ,stain are called gram negative'.Escherichia coli. It is gram —ye bacteria lives in intestinal tract of human beings and many other higher animals. E. coli is the best known prokaryotic cell. The growth characteristics of E. coli can be .
Aim of study

This paper describes the Anti-fungal and Bacterial analysis of compounds 4-(2-chloro-6-substituted¬quinolin-3-y1)-3-chloro-1-{443-(4-substituted phenyl)- acrylolyll-phenyl}-azetidine-2-ones.
Review of Literature

A fungi' lacking in chlorophyll, cannot synthesize their essential food requirements. They have therefore to be dependent of food already synthesized by other plants.

Bacteria,2'3 like fungi are devoid of chlorophyll with a saprophytic or parasitic mode of like and are unable to synthesize their food requirements from organic substances.

A large number of chalcones derivatives have been used as a bactericidal and fungicidal agents It was found that the chalcones derivatives have been found more active against4,5,6 8, enzymes4'5'6, bacteria , and fungi 9.
Methodology
In this method graded dilutions of the test compounds in a suitable nutrient medium are inoculated with the organism under examination using aseptic techniques and incubated under suitable conditions in an incubator. The minimum concentration of the compound preventing detectable growth (MIC) is taken and a measure of biocidal activity. In this method following steps are involved : (a) Sterilization of glass-ware (b) Preparation of culture media. (c) Sterilization of culture media (d) Preparation solution of New chalcones (e) Determination of MIC values
Result and Discussion

Table-4.1: Antimicrobial Activity of 4-(2-chloro-6-substituted­quinolin-3-y1)-3-chloro-1-{443-(4-substituted phenyl)- acrylolyll-phenyl}-azetidine-2-ones.

S.
No.

Compound

Anti-bacterial
MIC (1.1g/mL)

Anti-fungal
MIC (µg/mL)

Gram
Positive

Gram
Negative

S.
a ureus

E. coli

C
albicans

A.
niger

1

Ri-Ar

-

15

22

15

2

RI-Al.-CI (p)

10

14

23

-

3

R1-Ar-(Br) (p)

-

-

22

-

4

R1-Ar-Me (p)

-

19

20

21

5

R1-Ar-OMe (p)

19

19

20

22

6

R1-Ar-N (Me)2 (p)

-

20

12

-

7

R1-Ar-N(C2H5)2 (p)

16

-

14

16

Cont...Antimicrobial Activity of

S.
No.

Compound

Anti-bacterial
MIC (µg/mL)

Anti-fungal MIC (pg/mL)

Gram
Positive

Gram
Negative

S.
aureus

E. coli

C
albicans

A.
niger

8

R2-Ar

22

18

16

18

9

R2-Ar-C1 (p)

12

14

-

-

10

R2-Ar-(Br) (p)

21

23

21

23

11

R2-Ar-Me (p)

20

24

-

20

12

R2-Ar-OMe (p)

14

-

16

23

13

R2-Ar-N (Me)2 (p)

8

10

-

-

14

R2-Ar-N(C2H5)2 (p)

7

12

23

-

Cont...Antimicrobial Activity of

S.
No.

Compound

Anti-bacterial
MIC (ig/mL)

Anti-fungal
MIC (lg/mL)

Gram
Positive

Gram
Negative

S.
aureus

E. coli

C
albicans

A.
niger

15

R3-Ar

15

17

20

22

16

R3-Ar-C1 (p)

18

14

-24

-

17

R3-Ar-(Br) (p)

19

26

24

22

18

R3-Ar-Me (p)

21

-

20

17

19

R3-Ar-OMe (p)

12

10

12

11

20

R3-Ar-N (Me)2 (p)

8

10

10

12

21

R3-Ar-N(C2H5)2 (p)

-

18

19

23
Conclusion

From the result it is evident that most of the compound are very weekly active and few moderately active against Staphylococcus and Escherichia coli but compounds R3-Ar¬C1(p), R4-Ar-N(Me)2 (p), R4-Ar-N(C2H5)2 (p) possess very good activity against fungi A. niger and compound R3-Ar-OMe (p), R2-Ar-N (Me)2 (p), R2-Ar-N(C2H5)2 (p) compound showedmoderate activity all bacteria and fungi tested.
References

1. W.E. Autoline, J.M. Knight and D.H. Petering, J. Med. Chem., 19, 329 (1976).

2. T. Ramakrishna; J. Jackson and W.E. Levinson, Bio. Inorganic Chem., 9, 23 (1978).

3 E.S. Higgins, D.A. Richert and W.W. westerfeld; J. Nuture, 59, 539-559 (1956).

4. A.K.Sengupta and Madhry Goyal; J. Indian Chem. Soc., 60, 766 (1983).

5. R.J. Guest, Chem. Abst. 47, 76304g (1952).

6. S.R. Ames and C.A. Elvehjem; J. Biol Chem., 43, 165 (1946).

7. S. Turian; Z. Allg. Pathol; 14, 388 (1951).

8. M.L. Monaught and E.L. Owen; Intern. Conger. Biochem. Abstr. of communs. Ist. Congr. England, 304 (1949).

9. Manju Bala; J. of Asian Chem. Soc. 1 (1), (1992).