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Argemone mexicana L. and Argemone ochroleuca Sweet. are noxious weeds, which cause harm to agriculture, environment and human health. Several attempts have been made for prevention, eradication and control but the results are not so promising. However, Vermitechnology is the latest aspects of biotechnology where application of earthworm is made for combating the waste disposal problem for minimising the production effect and to get useful product “Vermicompost”.

Similar studies carried out by Biradar,D.P. (2006). Bionutrient potentiality of Parthenium hysterophorus and it’s utility of green manure in rice ecosystem, Sharma, V.Pandher, J.K. and Kanwar, .K. (2008), Biomanagement of Lantana (Lantana camara L.) and Parthenium hysterophorus L. through vermicomposting and it’s response on soil fertility, Sharma,Rajeev, Dwivedi, H.S. and Dwivedi, P.(2016). Utilization of three obnoxious weeds (Parthenium hysterophorus, Lantana camara and Eichhornia crassipes) through vermicomposting and their  response on vegetative growth of Soyabean crop , R. Ananthvalli et.al. (2019). Vermistabilization of sea weeds using an indigenous earthworm species Perionyx excavatus, C.Devi. et.al. (2020). Bioconversion of Lantana camara by vermicomposting with two different earthworm species in monoculture, H.Kauser. (2021). Fate of invasive weed Mikania micrantha Kunth using vermitechnology employing three monoculture of earthworm species, H.Kauser. Meena Khwairakpam(2022). Composting and vermicomposting of obnoxious weeds--A novel approach for degradation of allelochemicals.

The pot experiment was conducted during Rainy season. To prepare suitable feeding material for earthworms for production of vermicompost, plant parts were dried and mixed with soil and cow dung. This was kept for 15-20 days for partial degradation. This partially degraded material was taken for experiment study. Seven amendments were prepared for plant material.

Table 1. Amendments of plant material for vermicompost

Experiment No.	No. Of Replicates	Ratio Plant   :   Soil	Weight In Gram Plant  :  Soil
1	03	0      :    6   ( R1)	Nil    :   600
2	03	1      :    5   ( R2)	100  :   500
3	03	2      :    4   (R3)	200  :   400
4	03	3      :    3   (R4)	300  :   300
5	03	4     :     2   (R5)	400  :   200
6	03	5     :     1   (R6)	500  :   100
7	03	6     :     0   (R7)	600  :   Nil

Three pots of 9 inches in diameter were taken for each amendments and 25 mature local species of earthworms. Amynthus alexandri were transferred in each pot and allowed to grow. Regular water supply was maintained. After three months the experiment was terminated and the cast were collected separately and analyzed for chemical analysis.

pH and electrical conductivity of worm and determined in 1:5 soil solution of fresh sample using a digital pH meter and conductivity meter following methods of Jackson(1967) for the estimation of organic matter and nitrogen, worm cast was dried to constant weight in a hot air oven at 80 degree Celsius . Total organic matter was estimated by rapid titration method of Walkely and Black (1934) using diphenylamine as an indicator. Total nitrogen was determined by macro Kjeldahl method (Piper 1942). It was proceeded by digestion (in digesterTecator 2006), (in distillation unit), and titrated using 0.1N HCl. Available phosphorus was extracted with 0.002 N Sulphuric acid after stirring soil for half hour (Hesse 1971). The total phosphorus was determined by colorimetric method employing ammonium molybdate and stannous chloride. 

The data of the analysis of vermicompost are presented in table 2. pH values in seven amendments for Argemone varied between 6.6 to 7.2.

Electrical conductivity was found to be in the range of 0.57m mhos/cm to 2.02mhos/cm in Argemone. Electrical conductivity was 0.57, 0.64, 0.79, 1.04, 0.88, 1.74,2.02m mhos/ in seven amendments. Total phosphorus showed an increase / decrease pattern and varied between 0.00001 to 0.0001mg/litre%. As the values were 0.00001, 0.00004, 0.00003, 0.00006, 0.0001 and 0.00008mg/litre% for the seven amendments. The data for total organic matter (%) show an increase trend as plant material is increased in the amendments. It varied between 8.44% and 10.60% in Argemone. The values for seven amendments were 8.44, 8.51, 8.99, 9.33, 10.60, 9.02 and 10.08%.

The percentage of total nitrogen in seven amendments show a significant increase in the plant, it’s values were 0.32, 0.26, 0.37, 0.50, 0.60, 0.52, 0.43% for Argemone. Introduction of various local species for production of vermicast by using various earthworm species has gained importance for the composting of various animals and plants residues under semi- natural conditions. In this context a local species, Amynthus alexandri,was incorporated for the production of vermicompost by using Argemone plant species in different ratios. After three months of study it was observed that entire waste was converted into vermicompost, which was analysed for various parameters like pH, electrical conductivity,total phosphorus, nitrogen and total organic matter. Nogales et.al. (1998) studied degradation of dry olive oil cake through Eisenia anderi and the product obtained after 35 days was analysed for total organic carbon, total nitrogen, total phosphorus, pH and electrical conductivity etc.

The earthworm cast contains concentrated phosphorus, exchangeable magnesium, potassium and calcium, all essential for plant growth. The enrichment of earthworm casting in having essential plant nutrients such as N,K,P,Ca and Mg has been demonstrated by several workers (Lunt & Jacabson 1944, Parle 1963, Graff 1970 ). The present study also indicated that the earthworm castings of the plant material was richer organic carbon, nitrogen, and total phosphorus. Earthworms provide ideal conditions for nitrogen fixing bacteria and also discharge their nitrogenous waste into vermicompost (Bhawalker, 1992). Heine & Larink (1993) measured the cast production of Lumbricus terrestris, and analyzed it for Ca, P, and N. The content of Ca, P, and N in the cast was higher than the same amount of food. Nainawat (1997), Khandal (1999) ,Garg and Bhardwaj (2001) found that the casts produced by decomposition of kitchen waste, garden waste, municipal waste, terrestrial waste and aquatic weeds were also richer in these components, hence, confirming the results of the study. The data also revealed that the cast produced through vermicompost helps in maintaining the pH of soil that is chemically more neutral than the surrounding soil. It is because almost all the enzymes work at ideal pH between 6.3 and 7.3, which is responsible for maintaining Ph of the vermicompost (Bhiday 1992). Similarly total phosphorus, nitrogen and organic matter were also higher. The earthworms improve soil properties, augment the amount of mobile phosphorus and potassium (Atlavinyte et.al. 1975, Graff 1972 and Sokolov 1956) and increase the yield of plants (Atlavinyte et.al. 1968, Atlavinyte 1975 and Edwards 1980).

1. Atlavinyte, O. (1975). Ekologia dozdevych varvejich na plodorodie pocvyv,litovskoj SSR “ Mokaslas” vilnius.

2. Atlavinyte, O. Bagdonavicience, Z. and Budavicience, I.( 1968). The effect of lumbricidal on the barley crops in various soils. Pedobiologia 8: 415-423.

3. Bhawalkar, V.S. (1992). Vermiculture A promising source of biofertilizers, National Seminar on Agricultural Biotechnology, March, 7-8, 1989. Gujrat Agricultural University, Navsari India.

4. Bhiday, M. Bhawalkar, R. (1992). Agro- Ecology for sustainable future. The main issue, In L.L. Somani, K.L. Totawat and B.L. Baser (Eds.) Proceeding of national seminar on natural farming published by Rajasthan college of Agriculture. Pp 105-126.

5. Biradar, D.P., Shivkumar, K.S. Prakash, S.S. and Pujar, T. (2006). Bionutrient Potentiality of Parthenium hysterophorus and it’sutility of green manure in rice ecosystem. Karnataka Journal of Agricultural sciences. 19: 256-263.

6. C. ,Devi et.al. (2020) Bioconversion of Lantana camara by vermicomposting with two different earthworm species in monoculture, Bioresource Technology Vol. 296, Jan. 2020 122308.

7. Edwards, C.A.(1980). Earthworms soil fertility and plant growth. In.Applehof, M.(Ed.) Proceeding, Workshop on the role of earthworms in the stabilization of organic residues. Vol.1, Proceedings. Beech leaf press Kalamazoo, Michigan.

8.  Garg, Kavita and Bhardwaj Nagendra. (2001). Production of vermicompost through vermitechnology using two obnoxious weeds. Journal of environment and pollution 8: 49-52.

9. Graff, O., (1970). Phosphorus content of earthworm casts Land. Forsch. Volkenrode, 20: 33-36.

10. Graff, O.,(1972). Stickstoff, Phosphorus and calcium in der Regen auf der Wiesenversuchs flache des solling projekets. Ann Zool. Ecol. Anim. Num, H.Ser, 71:93-101.

11. H. Kauser, (2021). Fate of invasive weed Mikania micrantha Kunth using vermitechnology employing Bioresource Technology Reports Vol.  16, Dec. 2021, 100827

12. H.Kauser,Meena Khwairakpam, (2022). Composting and vermicompostingof obnoxious weeds --A novel approach for the degradation of allelochemicals In. book: Advanced organic waste Management (pp. 175-192).

13. Heine, O.and Larink, O. (1993). Food and cast analysis as a parameter of turnover of materials by earthworms (Lumbricus terrestris L.) Pedobiologia, 37: 245-256.

14. Hesse, P.R. (1971). A text book of Soil Chemical Analysis, John Murray, London.

15. Jackson, M.L. (1967). Soil chemical analysis, Prentice Hall India Pvt. Ltd. New Delhi.

16. Khandal, D.K. (1999). Vermitechnological study on aquatic weed, management. Ph.D. Thesis, University of Rajasthan, India.

17. Lunt, H.A. and Jacobson, G.M. (1944). The chemical composition of earthworm casts, Soil, Sci. 58:367-375. 

18. Nainawat, R.(1997). Vermitechnological studies on organic solid waste management, Ph. D . Thesis, University of Rajasthan, India.

19. Nogales, R., Thompson, R., Calmet, A., Benitez, E., Gomej, M.and Elvita, C.(1998). Feasibility of vermicomposting residues form olive oil production obtained using two stages centrifugation. Pp,1482-1506. In. J. Environ. Soil, Health, A33(7). Dpto. Agro, ecologia protection vegetal Estacionm. Experimental de Zaidin CSIC, Spain.

20. Parle, J.N.(1963). A microbiological study on earthworm casts, j.Gen. Microbio, (1963).

21. Piper, C.S. (1942). Soil and plant analysis, University of Adelaide, Australia.

22. Sharma, V. Pandher, J.K. and Kanwar, K. (2008). Biomanagement of Lantana (Lantana camara, L.) and Parthenium hysterophorus L. through vermicomposting and it’s response on soil fertility , Indian J. Agrc. Res.42 (4): 283-287. 

23. Sharma, Rajeev, Dwivedi, H.S. and Dwivedi, P. (2016).  Utilization of three obnoxious weeds (Parthenium hysterophorus, Lantana camara and Eichhornia crassipes) through vermicomposting and their response on vegetative growth of soyabean crop . International journal of Advanced Research in biological Sciences Vol. 3, issue- (9): 13-20.

24. Sokolov, A.A. (1956). Znacenies dozdevych cervej Vpoc Voob razovanii, Izd. A.N. Kazachsk, SSR. Alma Ara.

25. Walkeley, A.and Black, I.A.(1934). An examination of the degt jereff method for determining soil organic matter and prposed modification of chromic acid titration method, Soil Sci. 37: 29